Activated protein C resistance assay performance: Improvement by sample dilution with factor V-deficient plasma

From: Archives of Pathology & Laboratory Medicine | Date: May 1, 1998| Author: | Copyright information

Objectives.-To evaluate a modification of a commercially available reagent kit (COATEST APC Resistance Kit) for functional activated protein C (APC) resistance testing, and to determine the ability of the modified assay to demonstrate APC resistance in patients receiving warfarin.

Design.-Functional APC resistance testing was performed using both the first-generation COATEST APC Resistance Kit and a modified, or second-generation, version of the COATEST assay that uses predilution of the patient sample with factor V-deficient plasma. Factor V genotyping for APC resistance (FV R506Q) was ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Evaluation of recurrent thrombosis and hypercoagulability. (includes patient information)
; ...Causes of Hypercoagulability Inherited APC resistance (factor V Leiden) Protein S deficiency...connective tissue diseases, such as ANA D. APC resistance levels(*) E. Antigenic and functional...APC = activated protein C. (*)--Since APC resistance is the most common reason for ...
Editorial: Does thrombophilia cause perthes' disease in children
; ...However, only a half of the children with APC resistance, 12 in all, had a confirmatory polymerase...same group showed that the 'standard' APC resistance assay used to screen for the FVL mutation...factor VIII which can cause 'acquired' APC resistance, will not be detected, but at ...
Factor V Leiden in Blood Donors in Baghdad (Iraq)
; ...median, 30.5 years). A second-generation APC resistance test was performed on all included...case, resistant by second-generation APC resistance tests (clotting time, 39 s) was a non-carri...factor V gene maybe responsible for APC resistance in this particular case (6). The ...
Risk-factor profile in severe, generalized, obliterating vascular disease.(Case Rebort)
; ...for severe generalized OVD such as APC resistance; HHCE; and gene mutations of hemochromatosis...could not be detected in the patient. APC resistance is characterized by a poor anticoagulant...inactivating coagulation factors Va and VIIIa. APC resistance can be inherited or acquired ...
Bilateral thalamic infarction in a patient with factor V Leiden mutations
; ...1 The reported incidence of APC resistance due to this mutation is between 2% and 7%.2 Despite the role of APC resistance in venous thromboembolic disease...Further serologic testing revealed APC resistance, and polymerase chain reaction...
SYSTEMATIC ANALYSIS OF THROMBOPHILIA AFTER PREECLAMPSIA: PREVALENCE AND IMPACT ON SUBSEQUENT PREGNANCIES
; ...preecclampsia (252 with early-onset < 32 weeks) were examined 3 months postnatally for protein S and proteion C deficiency, APC resistance, factor V Leiden, prothrombin G20210A-mutation (Factor II), anticardiolipin antibodies, lupus anticoagulans, ATIII deficiency...
Activated Protein C Resistance and Factor V Leiden: A Review
; ...should be high in cases of unexplained venous thrombosis. APC resistance and FVL mutation can be diagnosed with high sensitivity...recognized that FVL accounts for 90% to 95% of cases of APC resistance. Factor V resistance to APC has an incidence of 4.8% in...
Feasibility of home monitoring of warfarin therapy in children. (adapted from the Journal of Pediatrics 1995;127:389-94)(Tips from Other Journals)
; ...should be used for monitoring patients receiving warfarin therapy The usual therapeutic range...whole blood monitors in children receiving warfarin therapy. Ninety-three children comprised...group included 40 children who were receiving warfarin and were followed in a hospital ...
Low-dose vitamin K may improve warfarin therapy.(Literature Review & Commentary)(Brief article)
; Eight patients (aged 45-79 years) receiving warfarin, whose international normalized ratios (INRs) had...which sometimes causes serious problems. Patients receiving warfarin must have their INR (a measure of blood clotting...
Warfarin can safely prevent stroke in AF patients over 75: prior RCT trials involved younger patients.(News)
; ...of death or major disabling stroke. The patient group receiving warfarin had 1.8 strokes per year, compared with 3.8 per year in...disabling stroke per year was prevented for every 50 patients receiving warfarin. A very important question is, was that at the expense...