Glutaraldehyde modified mica: A new surface for atomic force microscopy of chromatin

From: Biophysical Journal | Date: December 1, 2002| Author: Bash, Ralph; Et al; Hager, Gordon L; Yodh, Jiya G; Wang, Hongda | Copyright information

ABSTRACT We have found that mica surfaces functionalized with aminopropyltriethoxysilane and aldehydes bind chromatin strongly enough to permit stable and reliable solution imaging by atomic force microscopy. The method is highly reproducible, uses very small amounts of material, and is successful even with very light degrees of surface modification. This surface is far superior to the widely used aminopropyltriethoxysilane-derivatized mica surface and permits resolution of structure on the n...

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