Hepatitis C genotype determination by melting curve analysis with a single set of fluorescence resonance energy transfer probes

From: Clinical Chemistry | Date: December 1, 2002| Author: Bruns, David E; Haverstick, Doris M; Bullock, Grant C | Copyright information

Background: The genotype of hepatitis C virus (HCV) is a predictor of antiviral therapeutic response. We describe an approach for HCV genotype determination by real-time PCR and melting curve analysis.

Methods: After automated nucleic acid extraction, we used reverse transcription-PCR in a block cycler to amplify nucleotides 6-329 of the 5'-untranslated region of HCV. The product was further amplified by single-- tube real-time seminested PCR in a LightCycler(TM) instrument (Roche). T...

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