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Fractionation of Soybean Globulins Using Ca^sup 2+^ and Mg^sup 2+^: A Comparative Analysis
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Abstract
The substitution of CaCl^sub 2^ by MgCl^sub 2^ was undertaken in Deak's two-step process of separating the soybean 11S and 7S globulins, aiming at higher purities and lower phytic acid (PA) contents of recovered protein fractions. The effects of pH and the addition of NaCl were also evaluated. Compared with CaCl^sub 2^, MgCl^sub 2^ reduced the PA content of the 11S-rich fraction by 63-71% but increased that of the 7S-rich fraction by 14-28%, depending on pH. Correspondingly, more Ca^sup 2+^ was recovered in the 11S-rich fraction, while more Mg^sup 2+^ co-precipitated with the 7S-rich ...
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