Development of DNA aptamers for cytochemical detection of acetylcholine

In Vitro Cellular & Developmental Biology | March 1, 2008| | Copyright

Abstract This report describes a novel approach to the detection of acetylcholine using DNA aptamers. Aptamers were developed by eight rounds of acetylcholine affinity column chromatography and polymerase chain reaction (PCR) amplification. Sequences from rounds 5 and 8 were screened by colorimetric enzyme-based microtiter plate assays and found to bind acetylcholine and related compounds, but not unrelated compounds. One of the highest affinity aptamers, designated ACh 6R, was further tested in aptamer-peroxidase and aptamer-fluorescence staining protocols. Using Neuro-2a murine ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Anniversaries
Newspaper article from: The Independent - London ; ...Penrhyn Stanley, biographer, 1815; Ernst Werner von Siemens, inventor, 1816; Edwin George...Noel Paton, painter, 1821; Franz von Lenbach, painter, 1836; Lucien...Tartaglia, mathematician, 1557; Konrad von Gesner, physician and naturalist...
Birthdays & Anniversaries
Newspaper article from: The Independent - London ; ...Maximilien de Bethune, Duc de Sully, statesman, 1560; Heinrich (Harry) Heine, poet and journalist, 1797; Ernst Werner von Siemens, inventor, 1816. Deaths: Maimonides (Moses ben Maimon), Jewish philosopher, 1204; Donatello (Donato...
Friday, December 13
News Wire article from: AP Worldstream ; ...of the attackers are eventually killed. Today's Birthdays: Heinrich Heine, German poet (1797-1856); Ernst Werner von Siemens, German engineer (1816-1892); Emily Carr, Canadian painter/writer (1871-1945); Carlos Montoya...
Thursday, December 13
News Wire article from: AP Worldstream ; ...accused of supporting in attacks on civilians. Today's Birthdays: Heinrich Heine, German poet (1797-1856); Ernst Werner von Siemens, German engineer (1816-1892); Emily Carr, Canadian painter/writer (1871-1945); Carlos Montoya...
FirstAlert[tm] 4/29: Explosive Move Possible In S&P 500.
News Wire article from: Investrend ; ...Uma Thurman, Jay Cutler. Today in History: James Cook arrived at and named Botany Bay, Australia, in 1770. Ernst Werner von Siemens demonstrated the Elektromote, the forerunner of the trolleybus in Berlin in 1882. Gideon Sindback patented the...
Saturday, December 13
News Wire article from: AP Worldstream ; ...investigate September 11 terrorist attacks. Today's Birthdays: Heinrich Heine, German poet (1797-1856); Ernst Werner von Siemens, German engineer (1816-
Died on this day; Remember When.(Features)
Newspaper article from: Evening Chronicle (Newcastle, England) ; Byline: RAY MARSHAL 1882: Anthony Trollope. British novelist whose works include Barchester Towers 1892: Ernst Werner Von Siemens. German inventor. 1949: Huddie 'Leadbelly' Leadbetter. Known as the King of the Twelve String Guitar. 1973...
The disintegration of organised capitalism: German corporate governance in the 1990s.
Magazine article from: West European Politics ; ...traditionally played a prominent role in the German ideal of entrepreneurship. Industrial leaders of the past like Werner von Siemens, Ernst Abbe and Hugo Stinnes were admired both for their success in business and their public spirit. (4) The concept...
Siemens appoints new head of its office in Central Asia.
News Wire article from: UzReport ; ...2 (UzReport.com): Siemens Enterprise Communications...Grevtsev earlier worked for Ernst & Young, Pragma...Hamilton and Cisco. Siemens Enterprise Communications...than 160 years ago with Werner von Siemens and the invention of the...
Awards
Magazine article from: Manufacturing Engineering ; The Werner von Siemens Ring Stiftung (Foundation), which...Ditzingen, Germany), the Werner von Siemens Ring, which is described as Germany...three years to pioneers in technology. Ernst O. Gobel, president of the Physikalisch...

Find more facts and information related to the article "Development of DNA aptamers for cytochemical ..."