Site-directed mutagenesis of tyrosine 118 within the central constriction site of the LamB (Maltoporin) channel of Escherichia coli. II. Effect on maltose and...

From: Biophysical Journal | Date: July 1, 2002| Author: | Copyright information

ABSTRACT The 3-D structure of the maltooligosaccharide-specific LamB channel of Escherichia coli (also called maltoporin) is known from x-ray crystallography. The central constriction of the channel formed by the external loop 3 is controlled by tyrosine 118. Y118 was replaced by site-directed mutagenesis by 10 other amino acids (alanine (A), isoleucine (I), asparagine (N), serine (S), cysteine (C), aspartic acid (D), arginine (R), histidine (H), phenylalanine (F), and tryptophan (W))) including neutral ones, negatively and positively charged amino acids to study the effect of their size, ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Implication of virulence factors in Escherichia coli O157:H7 pathogenesis
Magazine article from: Critical Reviews in Microbiology ; ...outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 in 1982, numerous publications...gastrointestinal infection. Indeed, Escherichia coli O157:H7 pathogenesis is linked to...review I describe current knowledge of Escherichia coli O157:H7 pathogenesis with emphasis...
Plasmid profile of antibiotic resistant Escherichia coli isolated from chicken intestines.
Magazine article from: Journal of the Alabama Academy of Science ; ABSTRACT Fifty-seven strains of Escherichia coli from chicken intestines were isolated...profiling of antibiotic resistant Escherichia coli isolates revealed that the isolates...health (Chee-Sanford et al.., 2001). Escherichia coli strains isolated from sewage treatment...
Molecular characterization of shiga like toxin-producing Escherichia coli (STEC) isolates from pigs oedema
Magazine article from: Indian Journal of Medical Research ; ...pathogroups such as enteropathogenic Escherichia coli (EPEC), (eae/bfpA), enteroaggregative Escherichia coli (EAggEC), (eagg), enterotoxigive...and shiga like toxin producing Escherichia coli (STEC), (stx1/ stx2) by PCR and...
Genetic approaches to studying protein synthesis: Effects of mutations at (Psi)516 and A535 in Escherichia coli 16S rRNA
Magazine article from: The Journal of Nutrition ; ...Mutations at (Psi)516 and A535 in Escherichia coli 16S rRNA1,2 ABSTRACT A genetic system...mutations at the sole pseudouridine (1) in Escherichia coli 16S rRNA which is located at position...mutation in a ribosomal RNA operon of Escherichia coli. Proc. Nati. Acad. Sci. U.S.A. 79...
Caseinoglycomacropeptide Inhibits Adhesion of Pathogenic Escherichia coli Strains to Human Cells in Culture
Magazine article from: Journal of Dairy Science ; ...adhesion of 3 strains of verotoxigenic Escherichia coli (VTEC) and 3 strains of enteropathogenic Escherichia coli (EPEC) to human HT29 tissue cell...caseinoglycomacropeptide, bacterial adhesion, Escherichia coli, probiotic) Abbreviation key: CGMP...
Eiken Chemical to Launch LAMP-based Escherichia Coli O157 Reagent Test Kit
Newspaper article from: JCNN News Summaries - Japan Corporate News Network ; ...will begin marketing the Loopamp Escherichia coli O157 Reagent Test Kit, a reagent kit to detect Escherichia coli (E.coli)O157, on July 11. Using...Chemical expects that the Loopamp Escherichia coli O157 Reagent Test Kit will find...
The sialylated fraction of milk oligosaccharides is partially responsible for binding to enterotoxigenic and uropathogenic Escherichia coli human strains
Magazine article from: The Journal of Nutrition ; ...Enterotoxigenic and Uropathogenic Escherichia coli Human Strains1 ABSTRACT Milk oligosaccharides...CFA/II-expressing enterotoxigenic Escherichia coli (ETEC) strains constitute one of the...ability to bind two uropathogenic Escherichia coli (UPEC) strains was also examined...
Escherichia coli O157:H7: etiology, clinical features, complications, and treatment.
Magazine article from: Nephrology Nursing Journal ; In recent years, Escherichia coli (E. coli) 0157:H7 has developed...prevention of infection with Escherichia coli O157:H7 (E. coli O157:H7...association between enterohemmorhagic escherichia coli and hemolytic uremic syndrome...
Characterizing the growth of escherichia coli O157:H7 on the surface of beef.(COMMUNICATIONS--GRADUATE)(Clinical report)
Magazine article from: Proceedings of the North Dakota Academy of Science ; Escherichia coli O157:H7 has emerged as a major public...hot acid sprays to decontaminate Escherichia coli O157:H7 on beef. J. Food Prot. 57...infection by verotoxin-producing Escherichia coli O157:H7. J. Infect. Dis. 154:522...
Incidence of Escherichia coli O157:H7 and E. coli Virulence Factors in US Bulk Tank Milk as Determined by Polymerase Chain Reaction1
Magazine article from: Journal of Dairy Science ; ...associated with enterohemorrhagic forms of Escherichia coli (EHEC) using real-time and conventional...milk. Keywords: enterohemorrhagic Escherichia coli, foodborne disease, polymerase chain...Campylobacter spp., and pathogenic Escherichia coli are also present in dairy production...

Find more facts and information related to the article "Site-directed mutagenesis of tyrosine 118 within ..."