Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei1[W][OA] From: Plant Physiology | Date: May 1, 2008| Author: | Copyright American Society of Plant Biologists May 2008. Provided by ProQuest LLC. (Hide copyright information) Copyright information

We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

Multicellular eukaryotes exist in the form of complex ...

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

	Laser-capture microdissection, a tool for the global analysis of gene expression in specific plant cell types: Identification of genes expressed differentially in epidermal cells or vascular tissues of maize Magazine article from: Plant Cell ; ...Analysis of Gene Expression in Specific Plant Cell Types: Identification...High-resolution gene expression analysis of...individual cell types. At least...monitoring gene expression in specialized cell types. In mammals...
	A Gene Regulatory Network Model for Cell-Fate Determination during Arabidopsis thaliana Flower Development That Is Robust and Recovers Experimental Gene Expression Profiles(W) Magazine article from: Plant Cell ; ...states that match gene expression profiles observed...required to restrain cell types to those found...show that the cell types recovered depend...combination of gene expression states that specifies...lead to multiple cell types, each characterized...set ...
	Early gene expression associated with the commitment and differentiation of a plant tracheary element is revealed by cDNA-amplified fragment length polymorphism analysis Magazine article from: Plant Cell ; Early Gene Expression Associated with the Commitment and Differentiation...synchrony. To reveal the changing patterns of gene expression during the 48 h of transdifferentiation from mesophyll to TE cell fate, we used a cDNA-amplified fragment length...embryonic cells differentiate into the 40 ...
	Noninvasive Monitoring of Target Gene Expression by Imaging Reporter Gene Expression in Living Animals Using Improved Bicistronic Vectors Magazine article from: The Journal of Nuclear Medicine ; ...noninvasive imaging of therapeutic gene expression based on levels of reporter gene expression is a powerful tool to devise improved...IRES in mediating downstream reporter gene expression in cell culture and in vivo. Methods...
	Gene expression: better tools: more complex results.(Cover story) Magazine article from: Bioscience Technology ; Gene expression has become a mainstream technique...drug development. Broadly speaking, gene expression comes in two flavors: analytical...diseased/normal). With functional gene expression, the aim is to obtain some quantity...
	Tackling High Variability in Gene Expression Studies. Magazine article from: Genomics and Proteomics ; ...Guilherme J. M. Rosa High variability in gene expression studies often prevents differences...are enhancing the ability to measure gene expression. Each method poses unique limitations...suggestions to tackle high variability in gene expression profiling. Tools for measuring ...
	Toward a Systems Biology of Mouse Inner Ear Organogenesis: Gene Expression Pathways, Patterns and Network Analysis Magazine article from: Genetics ; ...most comprehensive study to date on gene expression during mouse inner ear (IE) organogenesis...specification. Additional, clear-cut, gene expression signatures marked specific structures...hearing loss. There are numerous other cell types in the IE that are also required ...
	Toward a Neutral Evolutionary Model of Gene Expression Magazine article from: Genetics ; ...that describes neutral changes of gene expression over evolutionary time as a compound...deviation from clock-like behavior of gene expression distances. Model-based analyses of gene expression profiles in primate liver and brain...
	MAIZEWALL. Database and Developmental Gene Expression Profiling of Cell Wall Biosynthesis and Assembly in Maize 1[W] Magazine article from: Plant Physiology ; ...for each entry and gene expression data that are accessible...cell wall-related gene expression in different organs...cell wall-related gene expression throughout the maize...involving specific gene family members and...diversity of lignified cell ...
	Use of serial analysis of gene expression technology to reveal changes in gene expression in Arabidopsis pollen undergoing cold stress1[w] Magazine article from: Plant Physiology ; ...characterized the global gene expression patterns of Arabidopsis...Serial Analysis of Gene Expression (SAGE). A total of...features of specific cell types are determined by their particular gene expression profiles. Such global gene expression patterns can ...

For more facts and information, see all results

Find more facts and information related to the article "Global Characterization of Cell-Specific Gene ..."