A Simple Assay to Measure Phagocytosis of Live Bacteria From: Clinical Chemistry | Date: May 1, 2008| Author: Bicker, Heike; HÃ ¶ flich, Conny; Wolk, Kerstin; Vogt, Katrin; Volk, Hans-Dieter; Sabat, Robert | Copyright American Association for Clinical Chemistry May 2008. Provided by ProQuest LLC.Copyright information

BACKGROUND: The phagocytosis of pathogens is essential for fighting infections. No assay is available, however, to measure both engulfment and degradation of bacteria under conditions similar to those in vivo. We sought to develop a flow cytometric assay to measure the engulfment and degradation of live bacteria by human blood monocytes and granulocytes.

METHODS: We generated enhanced green fluorescent protein (EGFP)-expressing Eschericha coli by transforming E. coli with the plasmid v...

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