Exon-Level Expression Profiling: A Comprehensive Transcriptome Analysis of Oral Fluids

From: Clinical Chemistry | Date: May 1, 2008| Author: Hu, Zhanzhi; Zimmermann, Bernhard G; Zhou, Hui; Wang, Jianghua; Henson, Bradley S; Yu, Weixia; Elashoff, David; Krupp, Guido; Wong, David T | Copyright information

BACKGROUND: The application of global gene expression profiling to saliva samples is hampered by the presence of partially fragmented and degraded RNAs that are difficult to amplify and detect with the prevailing technologies. Moreover, the often limited volume of saliva samples is a challenge to quantitative PCR (qPCR) validation of multiple candidates. The aim of this study was to provide proof-of-concept data on the combination of a universal mRNA-amplification method with exon arrays for ...

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