Genetic Variation in the [kappa]-Casein Gene (CSN3) of Chinese Yak (Bos grunniens) and Phylogenetic Analysis of CSN3 Sequences in the Genus Bos

From: Journal of Dairy Science | Date: March 1, 2008| Author: | Copyright information

ABSTRACT

Variants of κ-casein (CSN3) have been extensively studied in cattle and 13 alleles have been identified at the protein and DNA levels to date. Evolution of some of these alleles and a possible common ancestor remain unclear. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis of CSN3 exon IV in domesticated yak revealed a 2-allele polymorphism showing migration patterns different from known cattle variants. The PCR products of both yak CSN3 SSCP alleles were sequenced. All yak had nucleotide sequences coding for Thr in AA position 136 (identical ...

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