50 years ago in Biochemistry

The Scientist | May 1, 2008| | Copyright

Demystifying a Key Biochemical Reaction

As a young chemist at Columbia University in 1956, one of Robert Breslow's first research goals was to solve the mystery of how the enzyme thiamine pyrophosphate catalyzed biochemical reactions such as the Krebs cycle and photosynthesis. He used nuclear magnetic resonance to capture the subatomic properties of the reaction. Breslow can't recall exactly what he exclaimed when he first saw a hydrogen atom from the compound exchange with deuterium oxide. "Maybe I shouted 'Hot Dog!' I don't know." His findings, published in 1958, have been cited more than ...

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