Primer on medical genomics part II: Background principles and methods in molecular genetics From: Mayo Clinic Proceedings | Date: August 1, 2002| Author: Dewald, Gordon W; Et al; Whiteman, David A H; Wieben, Eric D; Tefferi, Ayalew | Copyright Mayo Foundation for Medical Education and Research Aug 2002. Provided by ProQuest LLC.Copyright information

Medical Genomics

The nucleus of every human cell contains the full complement of the human genome, which consists of approximately 30,000 to 70,000 named and unnamed genes and many intergenic DNA sequences. The double-helical DNA molecule in a human cell, associated with special proteins, is highly compacted into 22 pairs of autosomal chromosomes and an additional pair of sex chromosomes. The entire cellular DNA consists of approximately 3 billion base pairs, of which only 1 % is thou...

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	The Use of Real-Time Polymerase Chain Reaction for Rapid Diagnosis of Skeletal Tuberculosis Archives of Pathology & Laboratory Medicine ; We identified Mycobacterium tuberculosis DNA using real-time polymerase chain reaction on a specimen from an osteolytic lesion of a femoral condyle, in which the frozen section demonstrated granulomas. The process was much more rapid than is possible with culture. The rapid detection of M
	Generic polymerase chain reaction followed by DNA sequencing as a means of diagnosing bacteraemia Anaesthesia and Intensive Care ; SUMMARY There is increasing use of polymerase chain reaction techniques to diagnose infection. We report the use of polymerase chain reaction using a genetic section of the bacterial 16S rDNA gene-followed by nucleotide sequencing-to determine the species of the infecting bacteria. In the first
	Application of nested polymerase chain reaction (nPCR) using MPB 64 gene primers to detect Mycobacterium tuberculosis DNA in clinical specimens from extrapulmonary tuberculosis patients Indian Journal of Medical Research ; Background & objectives: The conventional culture technique for diagnosis of extrapulmonary tuberculosis is time consuming. In order to find a sensitive and rapid technique nested polymerase chain reaction (nPCR) targeting the conserved MPB 64 gene of Mycobacterium tuberculosis was evaluated
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