Comparative Genetics of Sex Determination: Masculinizing Mutations in Caenorhabditis briggsae From: Genetics | Date: March 1, 2008| Author: Kelleher, Danielle F; de Carvalho, Carlos Egydio; Doty, Alana V; Layton, Marnie; Cheng, Andy T; Mathies, Laura D; Pilgrim, Dave; Haag, Eric S | Copyright Genetics Society of America Mar 2008. Provided by ProQuest LLC.Copyright information

ABSTRACT

The nematodes Caenorhabditis elegans and C. briggsae independently evolved self-fertile hermaphroditism from gonochoristic ancestors. C. briggsae has variably divergent orthologs of nearly all genes in the C. elegans sex determination pathway. Their functional characterization has generally relied on reverse genetic approaches, such as RNA interference and cross-species transgene rescue and more recently on deletion mutations. We have taken an unbiased forward mutagenesis appr...