A novel in vitro three-dimensional skeletal muscle model From: In Vitro Cellular & Developmental Biology | Date: July 1, 2007| Author: Marquette, Michele L; Byerly, Diane; Sognier, Marguerite | Copyright Society for In Vitro Biology Jul/Aug 2007. Provided by ProQuest LLC.Copyright information

Abstract A novel three-dimensional (3D) skeletal muscle model composed of C2C12 mouse myoblasts is described. This model was generated by cultivating myoblasts in suspension using the rotary cell culture system (RCCS), a unique culture environment. Single-cell suspensions of myoblasts were seeded at 5×10^sup 5^/ml in growth medium without exogenous support structures or substrates. Cell aggregation occurred in both RCCS and suspension control (SC) conditions within 12 h but occurred more rap...