The Influence of the Loop between Residues 223-235 in Beetle Luciferase Bioluminescence Spectra: A Solvent Gate for the Active Site of pH-Sensitive Luciferases

From: Photochemistry and Photobiology | Date: January 1, 2008| Author: Arnoldi, Frederico G C; Barbosa, Joà Neto, Antonio J Silva; Ohmiya, Yoshihiro; £ o A R G; Viviani, Vadim R | Copyright information

ABSTRACT

Beetle luciferases emit a wide range of bioluminescence colors, ranging from green to red. Firefly luciferases can shift the spectrum to red in response to pH and temperature changes, whereas click beetle and railroadworm luciferases do not. Despite many studies on firefly luciferases, the origin of pH-sensitivity is far from being understood. Through comparative site-directed mutagenesis and modeling studies, using the pH-sensitive luciferases (Macrolampis and Cratomorphus di...

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