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DNA damage induced by mutagens in plant and human cell nuclei in acellular comet assay
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Abstract: Higher plant cells have a long tradition of use in the studies on environmental mutagenesis in situ, especially in relation to human health risk determination. The studies on the response of plant and human cells to physical and chemical mutagens showed differences in their sensitivity. The differences in the presence of cell components in plants and humans could influence such response. Additionally, the level of the organization of the employed material could influence DNA-damagin...
Related newspaper, magazine, and journal articles from HighBeam Research
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Investigating DNA Damage
The American Biology Teacher
; Adavances in the field of molecular biology, powered by a technological revolution, have increased dramatically over the past decades. Notable developments such as the cloning of adult sheep, the sequencing of the human genome, and the production of genetically modified organisms capture the
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Application of the Comet Assay for Investigation of Oxidative DNA Damage in Equine Peripheral Blood Mononuclear Cells1,2
The Journal of Nutrition
; ABSTRACT Oxidative stress occurs when antioxidant defense mechanisms are overwhelmed by free radicals and may lead to DNA damage, which has been implicated in processes such as aging and diseases such as cancer. The two main techniques presently used to quantify DNA damage are measurement of
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Test may point way to best cancer therapy
The Milwaukee Journal Sentinel
; Test may point way to best cancer therapy `Comet assay' of bladder tumor's resistance to radiation could help doctors decide the best treatment By IAN SAMPLE New Scientist Monday, February 21, 2000 Making tumor cells look like microscopic comets could save people with bladder cancer from the trauma
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The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C
Folia Histochemica et Cytobiologica
; Abstract: The comet assay, under neutral conditions, allows the assessment of DNA integrity influenced by sperm ageing, which is manifested in DNA double-strand breaks. Here, we attempted to use a modified neutral comet assay test (single-cell gel electrophoresis), to our knowledge for the first
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DNA Oxidation Products, Antioxidant Status, and Cancer Prevention1
The Journal of Nutrition
; EXPANDED ABSTRACT KEY WORDS: * antioxidant * biomarkers * cancer * DNA oxidation * 8-hydroxy-2-deoxyguanosine Oxygen is essential for life but can also harm cells. This is because active forms of oxygen such as hydroxyl (OH) and Superoxide (O2) radicals, hydrogen peroxide (H^sub 2^O^sub 2 and
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Induced and Photoinduced DNA Damage by Quinolones: Ciprofloxacin, Ofloxacin and Nalidixic Acid Determined by Comet Assay¶
Photochemistry and Photobiology
; ABSTRACT Quinolones are degraded by light with loss of their antimicrobial activity, generating active species or radicals. Evidence exists that some fluoroquinolones (lomefloxacin, fleroxacin and enoxacin) induce damage to the cellular membrane and DNA cleavage by photosensitization. In this
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Genotoxicity in Androscoggin River smallmouth bass
Northeastern Naturalist
; ABSTRACT - In order to better illuminate the genotoxic effects of a "complex mixture" of anthropogenic chemical toxins, we have employed the comet assay to measure the level of genotoxicity induced in a sentinel animal species, Micropterus dolomieu. Our analysis reveals a significant level of
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Interexperimental and Interindividual Variations of DNA Repair Capacities After UV-B and UV-C Irradiations of Human Keratinocytes and Fibroblasts
Photochemistry and Photobiology
; ABSTRACT DNA repair plays a central role in the cellular response to UV. In this work we have studied the response of skin cells (i.e. fibroblasts and keratinocytes) from the same or from different individuals after both ultraviolet-15 (UV-B) and ultraviolet-C (UV-C) irradiations using the comet
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Comet Assay.(EDITOR'S Choice: OCTOBER'S TECHNOLOGY)(Brief Article)
Bioscience Technology
; Comet Assay IV interactive video-based software system measures the DNA damage detected using single cell gel electrophoresis (SCGE). A high-definition video camera attached to a microscope provides real-time cell scoring by transferring a live video image to a computer screen with no time lag.
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Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage-Study on Cultured Melanocytes and Atypical Nevus Cells[dagger]
Photochemistry and Photobiology
; ABSTRACT Melanin synthesis is an oxygen-dependent process that acts as a potential source of reactive oxygen species (ROS) inside pigment-forming cells. The synthesis of the lighter variant of melanin, pheomelanin, consumes cysteine and this may limit the capacity of the cellular antioxidative
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