Characterization of human pituitary adenomas in cell cultures by light and electron microscopic morphology and immunolabeling From: Folia Histochemica et Cytobiologica | Date: April 1, 2005| Author: | Copyright Folia Histochemica et Cytobiologica 2005. Provided by ProQuest LLC.Copyright information

Abstract: The morphology and hormone production of pituitary adenoma cell cultures were compared in order to highlight their characteristic in vitro features. Cell suspensions were prepared from 494 surgical specimens. The 319 viable monolayer cultures were analyzed in detail by light microscopy and immunocytochemistry within two weeks of cultivation. Some cultures were further characterized by scanning, transmission and immunogold electron microscopy. The viability and detailed in vitro morphology of adenoma cells were found to be characteristic for the various types of pituitary tumors. The ...

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