Comprehensive Expression Profiling of Rice Grain Filling-Related Genes under High Temperature Using DNA Microarray[OA]

From: Plant Physiology | Date: May 1, 2007| Author: Yamakawa, Hiromoto; Hirose, Tatsuro; Kuroda, Masaharu; Yamaguchi, Takeshi | Copyright information

To elucidate the effect of high temperature on grain-filling metabolism, developing rice (Oryza sativa) 'Nipponbare' caryopses were exposed to high temperature (33°C/28°C) or control temperature (25°C/20°C) during the milky stage. Comprehensive gene screening by a 22-K DNA microarray and differential hybridization, followed by expression analysis by semiquantitative reverse transcription-PCR, revealed that several starch synthesis-related genes, such as granule-bound starch synthase I (GB...

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