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Multiple Functions of DNA Polymerases
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The primary role of DNA polymerases is to accurately and efficiently replicate the genome to ensure the maintenance of the genetic information and its faithful transmission through generations. This is not a simple task considering the size of the genome and its constant exposure to endogenous and environmental DNA damaging agents. Thus, a number of DNA repair pathways operate in cells to protect the integrity of the genome. In addition to their role in replication, DNA polymerases play a cen...
Related newspaper, magazine, and journal articles from HighBeam Research
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Multiple Functions of DNA Polymerases
Critical Reviews in Plant Sciences
; The primary role of DNA polymerases is to accurately and efficiently replicate the genome to ensure the maintenance of the genetic information and its faithful transmission through generations. This is not a simple task considering the size of the genome and its constant exposure to endogenous and
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DNA: Night at the Improv?
Jewish Exponent
; Jewish Exponent 08-26-2004 Billions of cells divide every day in our bodies to replace those that wear out. To be able to do so, their DNA must be copied. A new Weizmann Institute study shows that the molecules in charge of the task of copying DNA -- called DNA polymerases -- are able to improvise
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DNA Polymerases that Propagate the Eukaryotic DNA Replication Fork
Critical Reviews in Biochemistry and Molecular Biology
; ABSTRACT Three DNA polymerases are thought to function at the eukaryotic DNA replication fork. Currently, a coherent model has been derived for the composition and activities of the lagging strand machinery. RNA-DNA primers are initiated by DNA polymerase α-primase. Loading of the
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Roles of Arabidopsis AtREV1 and AtREV7 in Translesion Synthesis1
Plant Physiology
; Plants have mechanisms for repairing and tolerating detrimental effects by various DNA damaging agents. A tolerance pathway that has been predicted to be present in higher plants is translesion synthesis (TLS), which is catalyzed by polymerases. In Arabidopsis (Arabidopsis thaliana), however, the
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Enzyme and competent cell quality ensure cloning integrity: PCR fidelity is largely determined by the intrinsic error rate of DNA polymerases under the reaction conditions employed. This error rate can be minimized, in part, by the presence of an integral 3'-5' exonuclease activity, also called proofreading.(Application Note)
Bioscience Technology
; Whether the goal of a biological investigation is to sequence an entire genome, learn all there is to know about one protein, or decipher the regulation of a particular pathway, the integrity of early DNA amplification and cloning can determine a project's success. Two basic components of a cloning
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Rapid Changes in Plant Genomes
Plant Cell
; Beginning with Mendel, plant research has been the source of revolutionary discoveries that have changed our views of heredity. Therefore, baffling new observations in plant genetics that challenge current concepts continue a long tradition. One of these challenges comes from the study of heritable
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Sensitivity to Phosphonoacetic Acid: A New Phenotype to Probe DNA Polymerase [delta] in Saccharomyces cerevisiae
Genetics
; ... mismatch repair in yeast and human cells (TRAN et al 1999; GENSCHEL et al 2002; DZANTIEV ... which has a proofreading-defective DNA pol (TRAN et al 1999). MMR deficiency increased PAA ... pol2-4 (KoKOSKA et al. 1998; Xm et al. 1999; TRAN et al 1999). To determine if viability ...
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Highly Organized but Pliant Active Site of DNA Polymerase [beta]: Compensatory Mechanisms in Mutant Enzymes Revealed by Dynamics Simulations and Energy Analyses
Biophysical Journal
; ABSTRACT To link conformational transitions noted for DNA polymerases with kinetic results describing catalytic efficiency and fidelity, we investigate the role of key DNA polymerase [beta] residues on subdomain motion through simulations of five single-residue mutants: Arg-283-Ala, Tyr-271-Ala,
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Full DNA Polymerases.(NEW Technology)
Bioscience Technology
; Taq Full DNA Polymerases efficiently and conveniently amplify DNA from any template and are recommended for any general PCR amplification procedure. The DNA polymerase can generate the highest yields from any DNA template, including rare ones, from bacterial and plasmid DNA, cDNA and complex
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Inhibitory Action of Conjugated C^sub 18^-Fatty Acids on DNA Polymerases and DNA Topoisomerases
Lipids
; ABSTRACT: We reported previously that unsaturated linear-chain FA of the cis-configuration with a C^sub 18^-hydrocarbon chain such as linoleic acid (18:2Δ9c, 12c) could potently inhibit the activities of mammalian DNA polymerases and DNA topoisomerases, but their saturated forms could not.
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