|
PDE4 inhibitors attenuate fibroblast chemotaxis and contraction of native collagen gels
|
Therapies that mitigate the fibrotic process may be able to slow progressive loss of function in many lung diseases. Because cyclic adenosine monophosphate is known to regulate fibroblasts, the current study was designed to evaluate the activity of selective phosphodiesterase (PDE) inhibitors on two in vitro fibroblast responses: chemotaxis and contraction of three-dimensional collagen gels. Selective PDE4 inhibitors, rolipram and cilomilast, each inhibited the chemotaxis of human fetal lung ...
Related newspaper, magazine, and journal articles from HighBeam Research
|
|
PDE4 inhibitors attenuate fibroblast chemotaxis and contraction of native collagen gels
American Journal of Respiratory Cell and Molecular Biology
; Therapies that mitigate the fibrotic process may be able to slow progressive loss of function in many lung diseases. Because cyclic adenosine monophosphate is known to regulate fibroblasts, the current study was designed to evaluate the activity of selective phosphodiesterase (PDE) inhibitors on
|
|
Isolation of EpH4 mammary epithelial cell subpopulations which differ in their morphogenetic properties
In Vitro Cellular & Developmental Biology
; R. MONTESANO,1 J. V. SORIANO, I. FIALKA, AND L. ORCI SUMMARY EpH4 is a nontumorigenic cell line derived from spontaneously immortalized mouse mammary gland epithelial cells (Fialka et al., 1996). When grown in collagen gels, EpH4 cells give rise to different types of structures, e.g., solid cords
|
|
|
IN SITU COLLAGEN GELATION: A NEW METHOD FOR CONSTRUCTING LARGE TISSUE IN ROTARY CULTURE VESSELS
In Vitro Cellular & Developmental Biology
; SUMMARY In situ collagen gelation is a method that combines a static three-dimensional culture technique with rotating bioreactors. This method was designed for large dense tissue engineering ex vivo. To challenge the current limitations on size, we combined the static collagen gel embedding method
|
|
Diffusion and convection in collagen gels: Implications for transport in the tumor interstitium
Biophysical Journal
; ABSTRACT Diffusion coefficients of tracer molecules in collagen type I gels prepared from 0-4.5% w/v solutions were measured by fluorescence recovery after photobleaching. When adjusted to account for in vivo tortuosity, diffusion coefficients in gels matched previous measurements in four human
|
|
Cultural technique for in vitro modelling of prolonged bioadhesion
Biomedical Materials
; Pilot studies with the compound adhesive 'Orabase' adhering to the tissue showed no detrimental effects, with the adhesive remaining in place for 4-6 hours. The authors conclude that the system shows promise for the investigation of prolonged bioadhesion. Hamster cheek pouch mucosa was placed on
|
|
|
An improved method for the collagen gel contraction assay
In Vitro Cellular & Developmental Biology
; SUMMARY The collagen gel contraction (CGC) assay is used frequently to study the cell-mediated reorganization of the extracellular matrix. In a typical CGC assay, cells embedded in a disk-shaped lattice (gel) of native type I collagen fibers compress the fibers and, consequently, reduce the
|
|
|
Phosphodiesterase 4 inhibitor cilomilast inhibits fibroblast-mediated collagen gel degradation induced by tumor necrosis factor-(alpha) and neutrophil elastase
American Journal of Respiratory Cell and Molecular Biology
; Tissue destruction, resulting in emphysema, can be a consequence of several pathologic processes. The current study evaluated the effects of the phosphodiesterase (PDE)4 inhibitor, cilomilast, and other PDE inhibitors on the ability of fibroblasts to degrade extracellular matrix. Using the
|
|
|
Thrombin and TNF-[alpha]/IL-1[beta] Synergistically Induce Fibroblast-Mediated Collagen Gel Degradation
American Journal of Respiratory Cell and Molecular Biology
; Degradation of preexisting and newly synthesized extracellular matrix is thought to play an important role in tissue remodeling. The current study evaluated whether thrombin and TNF945;/IL-1β could collaboratively induce collagen degradation by human fetal lung fibroblasts (HFL-1) and adult
|
|
|
Persistence of (TGF-beta1) induction of increased fibroblast contractility
In Vitro Cellular & Developmental Biology
; SUMMARY Fibroblast contraction of collagen gels is regarded as a model of wound contraction. Transforming growth factor (TGF)-beta added to such gels can augment contraction consistent with its suggested role as a mediator of fibrotic repair. Since fibroblasts isolated from fibrotic tissues have
|
|
|
Reversible Cigarette Smoke Extract-Induced DNA Damage in Human Lung Fibroblasts
American Journal of Respiratory Cell and Molecular Biology
; Cigarette smoke contains thousands of chemicals, many of which may contribute to cytotoxicity and carcinogenesis. Using assays detecting DNA strand breaks (terminal transferase dUTP nick end labeling [TUNEL]) and DNA content (flow cytometry), we evaluated the genotoxic effect of cigarette smoke
|