Real-time PCR Assay for Ultrasensitive Quantification of DNA-Binding Proteins From: Clinical Chemistry | Date: April 1, 2007| Author: Hou, Peng; Chen, Zaozao; Ji, Meiju; He, Nongyue; Lu, Zuhong | Copyright American Association for Clinical Chemistry Apr 2007. Provided by ProQuest LLC.Copyright information

Background: The specific binding of proteins to DNA is a key step for many cellular activities, such as transcription regulation, DNA replication, recombination, repair, and restriction. The detection of DNA-binding proteins, as well as the identification of specific binding sites, is therefore important to understand gene expression mechanisms and cellular function. We describe an ultrasensitive method for quantification of DNA-binding proteins.

Methods: We combined the common exonucl...