Adventitious bud regeneration from leaf and cotyledon explants of Chinese hawthorn (Crataegus pinnatifida Bge. var. major N.E.Br.)

In Vitro Cellular & Developmental Biology | January 1, 2007| | Copyright

Abstract

Hawthorn (Crataegus spp.) is an important plant with a long history as an ornamental and a source of medicine. A protocol is outlined for adventitious bud regeneration from leaf and cotyledon explants of Chinese hawthorn (C. pinnatifida Bge. var. major N.E.Br.). Adventitious buds were induced on both the leaves of sprouting winter buds and the leaves of in vitro plants, but the percentage of bud regeneration from leaves of in vitro plants was very low-less than 6%. On N6 medium supplemented with 31.08 µM BA and 9.67 µM NAA, the percentages of bud regeneration from leaves of ...

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