Short Communication: Ability of Cultured Mammary Epithelial Cells in a Bicameral System to Secrete Milk Fat

From: Journal of Dairy Science | Date: February 1, 2007| Author: Clegg, R; Larondelle, Y; Schneider, Y-J; Ernens, I | Copyright information

ABSTRACT

Mammary epithelial cells from lactating cows were cultured onto inserts coated with type I collagen. Every second day, the rates of fatty acid synthesis and secretion were determined by measuring the amount of [^sup 14^C]-labeled sodium acetate incorporated into lipids over a 4-h period. The [^sup 14^C]-containing lipids were identified by thin layer chromatography fractionation. In parallel, the integrity of the cell layer was evaluated by measurement of transepithelial elect...

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