Shifts in Thioredoxin Reductase Activity and Oxidant Status in Mononuclear Cells Obtained from Transition Dairy Cattle

From: Journal of Dairy Science | Date: March 1, 2007| Author: | Copyright information

ABSTRACT

Measures of oxidative status were examined in 14 dairy cows during the transition period. Blood samples were obtained approximately 21 d before expected calving, at calving, and again at 21 d in milk (DIM). Plasma samples were used to determine lipid hydroperoxide concentrations. Total white blood cells were used to determine the oxidative status of glutathione. Peripheral blood mononuclear cell (PBMC) lysates were used to determine the total antioxidant potential and enzymatic activities of glutathione peroxidase (GPX) and thioredoxin reductase (TrxR1). Both plasma lipid ...

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