Photophysics and Photochemistry of Horseradish Peroxidase A2 upon Ultraviolet Illumination

From: Biophysical Journal | Date: March 15, 2007| Author: Carvalho, Ana Sofia Leità Et al; Klitgaard, Sà Petersen, Steffen B; £ ¸ Neves-Petersen, Maria Teresa; o; ren | Copyright information

ABSTRACT

Detailed analysis of the effects of ultraviolet (UV) and blue light illumination of horseradish peroxidase A2, a heme-containing enzyme that reduces H^sub 2^O^sub 2^ to oxidize organic and inorganic compounds, is presented. The effects of increasing illumination time on the protein's enzymatic activity, Reinheitzahl value, fluorescence emission, fluorescence lifetime distribution, fluorescence mean lifetime, and heme absorption are reported. UV illumination leads to an exponen...

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