The Multicolumn Countercurrent Solvent Gradient Purification Process From: Biopharm International | Date: February 1, 2007| Author: Aumann, Lars; Mà Morbidelli, Massimo; Tarafder, Abhijit; ¤ ¶ ¼ hlein, Guido; ller-Spà Strà th, Thomas | Copyright Advanstar Communications, Inc. Feb 2007. Provided by ProQuest LLC.Copyright information

A continuous chromatographic process for monoclonal antibodies without using Protein A

ABSTRACT

This article presents the multicolumn countercurrent solvent gradient purification (MCSGP) process, which uses three chromatographic columns, and incorporates the principle of countercurrent operation and the possibility of using solvent gradients. A MCSGP prototype has been built using commercial chromatographic equipment. The application of this prototype for purifying a MAb from a ...

Related newspaper, magazine, and journal articles from HighBeam Research

	The Multicolumn Countercurrent Solvent Gradient Purification Process Biopharm International ; A continuous chromatographic process for monoclonal antibodies without using Protein A ABSTRACT This article presents the multicolumn countercurrent solvent gradient purification (MCSGP) process, which uses three chromatographic columns, and incorporates the principle of countercurrent operation
	Carbohydrate analysis columns. (New Products). Bioscience Technology ; Prevail[TM] Carbohydrate ES column provides carbohydrate analysis by HPLC. It offers high efficiency at ambient temperature, long column life, single peaks from anomeric sugars, solvent gradient compatibility, stable detector baselines, and the ability to change mobile phase composition. Alltech
	Using High Temperature HPLC for Improved Analysis Pharmaceutical Technology Europe ; Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the relationship between temperature and viscosity strongly influences the mobile phase flow profile and
	SFC Preparative package includes 5-cm column.(PRODUCT NEWS) Laboratory Equipment ; The SFC Prep 350 and 5-cm column package reduces time and operating costs by incorporating an automated C[O.sub.2] recycling feature. This makes it suitable for purification process development and laboratory and turnkey industrial solutions. The automated system features co-solvent gradient, high
	Only on M.E. Magazine Online: @ www.memagazine.org. Mechanical Engineering-CIME ; ... site 15% Note: Table made from pie chart. The June Question of the Month is: Where do you find most of your engineering-related news? The next Question of the Month will be posted on June 1. The Mechanical Engineering Magazine Online exclusive feature article ...
	Identification and Quantification of Feruloylated Mono-, Di-, and Triacylglycerols from Vegetable Oils JAOCS, Journal of the American Oil Chemists' Society ; ABSTRACT: The use of HPLC-MS to separate and identify the feruloylated acylglycerols formed during the transesterification of ethyl ferulate with TAG was examined. Novozym 435 (Candida antarctica lipase B)-catalyzed transesterifications of ethyl ferulate and soybean oil resulted in a mixture of
	Hesperetin glucuronide, a photoprotective agent arising from flavonoid metabolism in human skin fibroblasts[para] Photochemistry and Photobiology ; ABSTRACT There is considerable interest in the biological properties of flavonoids in terms of their antioxidant and cytoprotective actions. The interaction of the flavanone hesperetin with human skin fibroblasts (FEK4) has revealed the potential for metabolism to hesperetin glucuronide and its
	Chromatographic and spectroscopic methods in the analysis of triacylglycerol species and regiospecific isomers of oils and fats Critical Reviews in Food Science and Nutrition ; ABSTRACT: The chromatographic, spectrophotometric, and spectroscopic methods usually used for the analysis of oils and fats are reviewed, with special attention to the detection, identification, and quantitation of the triacylglycerol species present in the most common edible vegetable oils. Also,
	The future of liquid chromatography? Chemistry and Industry ; Familiar in one guise or another to every chemist, chromatography is probably the most used tool for separating liquids, but is this technique about to change? Untangling the mysteries of a chemical mixture is an everyday problem for the analytical chemist, and there is an impressive battery of
	Bringing Meaning to Pharmaceutical High-Throughput LC/MS.(research)(Brief Article) R & D ; The most frequent use of mass spectrometers in the pharmaceutical industry is to measure molecular mass--quickly, reliably, accurately to 0.1 dalton or so, and over and over again. High-throughput screening (HTS) of millions of compounds per year generates the leads that ultimately become

See all results.