Dynamics of the Nucleotide Pocket of Myosin Measured by Spin-Labeled Nucleotides

Biophysical Journal | January 1, 2007| | Copyright

ABSTRACT

We have used electron paramagnetic probes attached to the ribose of ATP (SL-ATP) to monitor conformational changes in the nucleotide pocket of myosin. Spectra for analogs bound to myosin in the absence of actin showed a high degree of immobilization, indicating a closed nucleotide pocket. In the Actin.Myosin.SL-AMPPNP, Actin.Myosin.SL-ADP.BeF^sub 3^, and Actin.Myosin.SL-ADP.AIF^sub 4^ complexes, which mimic weakly binding states near the beginning of the power stroke, the nucleotide pocket remained closed. The spectra of the strongly bound Actin.Myosin.SL-ADP complex consisted of two ...

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