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Lateral Flow Immunoassay Using Europium (III) Chelate Microparticles and Time-Resolved Fluorescence for Eosinophils and Neutrophils in Whole Blood
From:
Clinical Chemistry
| Date:
February 1, 2007| Author:
Jonsson, Ann; MÃ Mendel-Hartvig, Ib; Venge, Per; ¥ ¶ m, Gerd; rtensson, Ola; RundstrÃ
| Copyright American Association for Clinical Chemistry Feb 2007. Provided by ProQuest LLC.Copyright information
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Background: A simple point-of-care method for measuring leukocyte counts in a doctor's office or emergency room could be of great importance. We developed a protocol for measuring cell count by disrupting the cell membrane and analyzing specific proteins within the cells and used it to analyze proteins from eosinophils and neutrophils.
Methods: Lateral immunochromatographic (ICR) assays have been developed for eosinophil protein X (EPX) and human neutrophil lipocalin (HNL) as measures ...