Rice TATA binding protein interacts functionally with transcription factor IIB and the RF2a bZIP transcriptional activator in an enhanced plant in vitro transcription system From: Plant Cell | Date: April 1, 2002| Author: Zhu, Qun; Ordiz, Maria Isabel; Dabi, Tsegaye; Beachy, Roger N; Lamb, Chris | Copyright American Society of Plant Physiologists Apr 2002. Provided by ProQuest LLC.Copyright information

TATA binding protein (TBP) and transcription factor IIB (TFIIB) are key factors for the assembly of eukaryotic transcription initiation complexes. We used a rice whole-cell extract in vitro transcription system to characterize the functional interactions of recombinant plant TBP and TFIIB. Bacterially expressed rice TBP (OsTBP2) bound to the TATA box of the rice pal gene encoding phenylalanine ammonia-lyase, caused DNA bending, and enhanced basal transcription from the pal promoter in a TATA ...