Model-Based Analysis for Kinetic and Equilibrium Investigations

From: Critical Reviews in Analytical Chemistry | Date: January 1, 2006| Author: Maeder, Marcel; Norman, Sarah | Copyright information

Equilibrium and kinetic data usually can be described quantitatively by a chemical model that is based on the law of mass action. In such instances parameters of interest like rate and equilibrium constants and, depending on the nature of the data, also spectral information can be determined by model-based analysis of the appropriate data sets. In this contribution the essential aspects of the complete process are discussed, these include data acquisition, modelling of the concentration profi...

Related newspaper, magazine, and journal articles from HighBeam Research

Model-Based Analysis for Kinetic and Equilibrium Investigations
Critical Reviews in Analytical Chemistry ; Equilibrium and kinetic data usually can be described quantitatively by a chemical model that is based on the law of mass action. In such instances parameters of interest like rate and equilibrium constants and, depending on the nature of the data, also spectral information can be determined by
Ionic strength dependence of localized contact formation between membranes: Nonlinear theory and experiment
Biophysical Journal ; ABSTRACT Erythrocyte membrane surface or suspending phase properties can be experimentally modified to give either spatially periodic local contacts or continuous contact along the seams of interacting membranes. Here, for cells suspended in a solution of the uncharged polysaccharide dextran, the
Probing the electrostatic shielding of DNA with capillary electrophoresis
Biophysical Journal ; ABSTRACT The free solution mobility of a 20-bp double-stranded DNA oligomer has been measured in diethylmalonate (DM) and Tris-acetate buffers, with and without added NaCl or TrisCl. DM buffers have the advantage that the buffering ion is anionic, so the cation composition in the solution can be
Direct Measurement of Equilibrium Constants for High-Affinity Hemoglobins
Biophysical Journal ; ABSTRACT The biological functions of heme proteins are linked to their rate and affinity constants for ligand binding. Kinetic experiments are commonly used to measure equilibrium constants for traditional hemoglobins comprised of pentacoordinate ligand binding sites and simple bimolecular reaction
A simple method for determination of volatile acid concentration with corrections for ionic strength
Water Environment Research ; ABSTRACT: A relatively simple but accurate method to determine volatile acid concentration in aqueous solutions is presented. This new method extends a less accurate procedure previously proposed to account for ionic strength and combines several volatile acid concentrations into a composite value.
Electrostatic Steering at Acetylcholine Binding Sites
Biophysical Journal ; ABSTRACT The electrostatic environments near the acetylcholine binding sites on the nicotinic acetylcholine receptor (nAChR) and acetylcholinesterase were measured by diffusion-enhanced fluorescence energy transfer (DEFET) to determine the influence of long-range electrostatic interactions on
The iso-competition point for counterion competition binding to DNA: Calculated multivalent versus monovalent cation binding equivalent
Biophysical Journal ; ABSTRACT In this paper we introduce an important parameter called the iso-competition point (ICP), to characterize the competition binding to DNA in a two-cation-species system. By imposing the condition of charge neutralization fraction equivalence theta^sub 1^ = Ztheta^sub z^ upon the two
Ca2+ and Ionic Strength Dependencies of S1-ADP Binding to Actin-Tropomyosin-Troponin: Regulatory Implications
Biophysical Journal ; ABSTRACT Skeletal and cardiac muscle contraction are inhibited by the actin-associated complex of tropomyosin-troponin. Binding of Ca2+ to troponin or binding of ATP-free myosin to actin reverses this inhibition. Ca2+ and ATP-free myosin stabilize different tropomyosin-actin structural
Electrostatic Interactions Modulate the Conformation of Collagen I
Biophysical Journal ; ABSTRACT The pH- and electrolyte-dependent charging of collagen I fibrils was analyzed by streaming potential/streaming current experiments using the Microslit Electrokinetic Setup. Differential scanning calorimetry and circular dichroism spectroscopy were applied in similar electrolyte solutions
Ionic interactions play a role in the regulatory mechanism of scallop heavy meromyosin
Biophysical Journal ; ABSTRACT Heavy meromyosin from scallop (scHMM) striated muscle is regulated by calcium binding to the essential light chain. The regulation can be modeled with a calcium-dependent equilibrium between on and off scHMM conformations. The observed rate constant for mant-ADP dissociation from scHMM is