In Vitro propagation of the medicinal plant Plumbago Zeylanica L. through nodal explants

In Vitro Cellular & Developmental Biology | March 1, 2001| | Copyright

SUMMARY

A protocol for rapid in vitro propagation using nodal explants obtained from 2-yr-old, field-grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae is described. High frequency bud break and fast development of shoots were induced on Murashige and Skoog's basal medium supplemented with 27.2 (mu)M adenine sulfate +2.46 (mu)M indole-3-butyric acid (IBA). Induction of rooting was achieved by transferring the shoots to the same basal medium containing 4.92 (mu)M IBA. Using our protocol from one twig of P. zeylanica (eight responsive nodes per explant ...

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