Microbial hazards in plant tissue and cell cultures

In Vitro Cellular & Developmental Biology | March 1, 2001| | Copyright

(Received 18 August 2000; accepted 27 September 2000; editor T.A. Thorpe)

A wide range of microorganisms (filamentous fungi, yeasts, bacteria, viruses and viroids) and micro-arthropods (mites and thrips) have been identified as contaminants in plant tissue cultures. Contaminants may be introduced with the explant, during manipulations in the laboratory or by micro-arthropod vectors. Contaminants may express themselves immediately or can remain latent for long periods of time. This often makes it difficult to identify the source of contamination. Disinfection protocols have now been developed ...

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