Nucleic acid molecular biomarkers for diagnostic biodosimetry applications: Use of the fluorogenic 5'-nuclease polymerase chain reaction assay

From: Military Medicine | Date: February 1, 2002| Author: | Copyright information

A reliable, relatively easy method for diagnostic assessment of radiation exposure is needed to support the triage of radiation casualties and medical treatment decisions in military defense operations. Our strategy is to identify radiation-responsive DNA mutations and gene expression targets that can be analyzed using polymerase chain reaction (PCR) assays and an existing fluorescence-based nucleic acid analysis system designed for forward-deployable laboratory applications. Using an in vitro model system of human peripheral blood lymphocytes, we identified a candidate nucleic acid biomarker ...

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