Nicotinic Acetylcholine Receptor Channel Electrostatics Determined by Diffusion-Enhanced Luminescence Energy Transfer
From: Biophysical Journal
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Date: 8/15/2006
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Author: Lurtz, Monica M; Pedersen, Steen E; Wensel, Theodore G; Meltzer, Robert H
ABSTRACT
The electrostatic potentials within the pore of the nicotinic acetylcholine receptor (nAChR) were determined using lanthanide-based diffusion-enhanced fluorescence energy transfer experiments. Freely diffusing Tb^sup 3+^-chelates of varying charge constituted a set of energy transfer donors to the acceptor, crystal violet, a noncompetitive antagonist of the nAChR. Energy transfer from a neutral Tb^sup 3+^-chelate to nAChR-bound crystal violet was reduced 95% relative to the energy ...
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Copyright Biophysical Society Aug 15, 2006
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