The Use of Real-Time Polymerase Chain Reaction for Rapid Diagnosis of Skeletal Tuberculosis

From: Archives of Pathology & Laboratory Medicine | Date: July 1, 2006| Author: Bauer, Thomas W; Et al; Fraser, Thomas G; Joyce, Michael J; Kobayashi, Naomi | Copyright information

We identified Mycobacterium tuberculosis DNA using real-time polymerase chain reaction on a specimen from an osteolytic lesion of a femoral condyle, in which the frozen section demonstrated granulomas. The process was much more rapid than is possible with culture. The rapid detection of M tuberculosis and the concomitant exclusion of granulomatous disease caused by nontuberculous mycobacteria or systemic fungi are necessary to appropriately treat skeletal tuberculosis. The detection and ident...

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