Uptake of a fluorescent marker in palnt cells is sensitive to Brefeldin A and Wortmannin

From: Plant Cell | Date: January 1, 2002| Author: | Copyright information

We assessed FM1-43 [N-(3-triethylammoniumpropyl)-4-(4-[dibutylamino]styryl)pyridinium dibromide] as a fluorescent endocytosis marker in intact, walled plant cells. At VC, FM1-43 stained the plasma membrane, and after 30 to 120 min of incubation at 26 deg C, FM1-43 labeled cytoplasmic vesicles and then the vacuole. Fluorimetric quantitation demonstrated dye uptake temperature sensitivity (~65% reduction at 16 deg C, >90% at 4 deg C). FM1-43 uptake in suspension cells was stimulated more than twofold by brefeldin A and inhibited ~0.4-fold by wortmannin. FM1 -43 delivery to the vacuole was ...

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