Hydrolysis Methods Impacts Recovery and Detection of Urine Opiates

Clinical Chemistry | June 1, 2006| | Copyright

B-122

P. Wang. J. Ston4, K. Chen, A. Wu. UCSF/San Francisco General Hospital, Sun Francisco, CA.

Background: Confirmation of opiates in clinical urine samples is usually done by gas chromalography coupled with mass speclromelry (GCMS). There are several different sample preparation procedures in use. In these procedures, opiates are extracted from urine with hydrolysis to liberate drugs from their glueuronide conjugates. Both acid hydrolysis and enzyme hydrolysis using β-glucuronidase have been reported, with the latter prevailing in most clinical laboratories. Studies have shown these ...

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