Intragenic Suppression of Gal3^sup C^ Interaction With Gal80 in the Saccharomyces cerevisiae GAL Gene Switch

From: Genetics | Date: January 1, 2006| Author: Bewley, Maria; Et al; Peng, Gang; Pilauri, Vepkhia; Diep, Cuong Q | Copyright information

ABSTRACT

Gal4-mediated activation of GAL gene transcription in Saccharomyces cerevisiae requires the interaction of Gal3 with Gal80, the Gal4 inhibitor protein. While it is known that galactose and ATP activates Gal3 interaction with Gal80, neither the mechanism of activation nor the surface that binds to Gal80 is known. We addressed this through intragenic suppression of GAL3^sup C^ alleles that cause galactose-independent Gal3-Gal80 interaction. We created a new allele, GAL^sup SOC^,...

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