Processivity Clamp gp45 and ssDNA-Binding-Protein gp32 Modulate the Fidelity of Bacteriophage RB69 DNA Polymerase in a Sequence-Specific Manner, Sometimes Enhancing and Sometimes Compromising Accuracy

From: Genetics | Date: March 1, 2005| Author: Carver, Geraldine T; Drake, John W; Kadyrov, Farid A; Kissling, Grace E; Bebenek, Anna | Copyright information

ABSTRACT

Numerous studies of the impact of accessory proteins upon the fidelity of DNA synthesis have provided a complex and sometimes discordant picture. We previously described such an analysis conducted in vitro using various bacteriophage RB69 gp43 mutator DNA polymerases with or without the accessory proteins gp32 (which binds single-stranded DNA) plus gp45/44/62 (processivity clamp and its loaders). Mutations were scored at many sites in the lacZα mutation reporter sequence....

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