PRODUCTION OF PILOCARPINE IN CALLUS OF JABORANDI (PILOCARPUS MICROPHYLLUS STAPF)

In Vitro Cellular & Developmental Biology | November 1, 2005| | Copyright

SUMMARY

Jaborandi (Pilocarpus microphyllus) is the only known source of pilocarpine, and although this alkaloid is the only natural compound used to treat glaucoma, very little is known about its metabolism. Calluses obtained from petioles of P. microphyllus leaves were partially immersed in MS (Murashige and Skoog) liquid medium containing different pH levels (4.8, 5.8, and 6.8), nutrient concentration (MS normal basal medium concentration, absence of N, P, and K and three times normal concentrations), histidine and threonine (0.05, 0.15, and 0.75mM), NaCl (25 and 75 mM) and polyethylene ...

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