A human dendritic cell-based method to identify CD[4.sup.+] T-cell epitopes in potential protein allergens. (Mini-Monograph). Environmental Health Perspectives | February 1, 2003| | COPYRIGHT 2003 National Institute of Environmental Health Sciences. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information) Copyright

We developed an assay to determine the location of immunodominant CD[4.sup.+] T-cell epitopes in any protein. The method uses CD[4.sup.+] T cells from community donors in conjunction with dendritic cells derived in vitro. Synthetic peptides constructed to describe the sequence of the protein of interest are cocultured with dendritic cells and CD[4.sup.+] T cells, and T-cell proliferation is measured. Data are compiled over a large replicate of human donors to pinpoint immunodominant, usually promiscuous epitope regions. We have applied this technique to a known food allergen, the ...

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