Y-chromosomal DNA markers for discrimination of chemical substance and effluent effects on sexual differentiation in salmon. (Articles).

From: Environmental Health Perspectives | Date: September 1, 2002| Author: Afonso, Luis O.B.; Devlin, Robert H.; Ikonomou, Michael G.; Smith, Jack L. | Copyright information

Chinook salmon alevins were exposed during their labile period for sex differentiation to different concentrations of bleached kraft mill effluent (BKME), primary sewage effluent, secondary sewage effluent (SE), 17[beta]-estradiol, testosterone, and nonylphenol. After exposure for 29 days post hatching (DPH), fish were allowed to grow until 103 and 179 DPH, at which time their genetic sex was determined using Y-chromosomal DNA markers and their gonadal sex was determined by histolo...

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