Short interfering RNA (siRNA) Transfection Reagent allows for efficient target gene knock out. (Tools & Techniques).

From: Bioscience Technology | Date: September 1, 2002| Author: | Copyright information

The ability to efficiently knock out target gene expression will have a tremendous impact on the study of molecular and cellular processes. Unfortunately, the use of classical anti-sense techniques employing various chemically modified forms of oligonucleotides often results in insufficient or non-specific suppression of gene expression. It has recently been shown that the presence of long double-stranded RNA (dsRNA) induces RNA interference in a diverse group of organisms as well as cells in culture. RNA interference leads to the inhibition of protein expression via the ...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and trade journal articles from HighBeam Research

(Including press releases, facts, information, and biographies)

Stabilization of gene expression profiles in blood after phlebotomy
; Stabilization of Gene Expression Profiles in Blood...clinical samples. Gene expression profiling of...produce changes in gene expression ex vivo, e.g...If changes in gene expression occur after phlebotomy...analyze changes in gene expression in human ...
Noninvasive Monitoring of Target Gene Expression by Imaging Reporter Gene Expression in Living Animals Using Improved Bicistronic Vectors
; ...noninvasive imaging of therapeutic gene expression based on levels of reporter gene expression is a powerful tool to devise improved...IRES in mediating downstream reporter gene expression in cell culture and in vivo. Methods...
Gene expression: better tools: more complex results.(Cover story)
; Gene expression has become a mainstream technique...drug development. Broadly speaking, gene expression comes in two flavors: analytical...diseased/normal). With functional gene expression, the aim is to obtain some quantity...
Tackling High Variability in Gene Expression Studies.
; ...Guilherme J. M. Rosa High variability in gene expression studies often prevents differences...are enhancing the ability to measure gene expression. Each method poses unique limitations...suggestions to tackle high variability in gene expression profiling. Tools for measuring ...
Toward a Neutral Evolutionary Model of Gene Expression
; ...that describes neutral changes of gene expression over evolutionary time as a compound...deviation from clock-like behavior of gene expression distances. Model-based analyses of gene expression profiles in primate liver and brain...
Use of serial analysis of gene expression technology to reveal changes in gene expression in Arabidopsis pollen undergoing cold stress1[w]
; ...have characterized the global gene expression patterns of Arabidopsis pollen using Serial Analysis of Gene Expression (SAGE). A total of 21,237 SAGE...determined by their particular gene expression profiles. Such global gene expression...
High-Throughput RNA Isolation Technologies. New Tools for High-Resolution Gene Expression Profiling in Plant Systems1
; THE CHALLENGE OF DISSECTING GENE EXPRESSION IN MULTICELLULAR ORGANISMS The complete...addition to hybridizationbased measurements of gene expression, sequencing, or PCR-based gene expression analyses are being used. Available expression...
Adding Confidence to Gene Expression Clustering
; ...It has been well established that gene expression data contain large amounts of random...dissimilarity function to ascertain gene expression clusters and conditional randomization...statistically significant clusters of gene expression patterns, we aim to provide a level...
Oxidants, Antioxidants, and Modification of Gene Expression
; ...Antioxidants, and Modification of Gene Expression. Malcolm J. Jackson. School of Clinical...recognition that redox regulation of gene expression may be important in multiple processes...modulate ROS-stimulated changes in gene expression. In addition, evidence is increasing...
Universal RNA Reference Materials for Gene Expression
; ...Metrology and Standards Needs for Gene Expression Technologies: Universal RNA Standards...that would help in standardization of gene-expression technologies: an Assay Process Reference...underway at NIST and among members of the gene expression array community to further define...