rpoB Gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism. (Research).(Statistical Data Included)

From: Emerging Infectious Diseases | Date: November 1, 2001| Author: Arenas-Huertero, Catalina; Bobadilla-del-Valle, Miriam; Couary, Patricia; Kato-Maeda, Midori; Ponce-de-Leon, Alfredo; Ruiz-Palacios, Guillermo M.; Sifuentes-Osornio, Jose; Small, Peter M.; Vargas-Alarcon, Gilberto | Copyright information

The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using the DNA sequencing of the core region of rpoB for comparison. Of the RIFr, 24 had a PCR-SSCP pattern identical to that of H37Rv; the other 11 had four ...

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