Novel Method for Simultaneous Analysis of p53 and K-ras Mutations and p53 Protein Expression in Single Histologic Sections. From: Archives of Pathology & Laboratory Medicine | Date: March 1, 2001| Author: Okayasu, Isao; Shinoda, Hiroshi; Yamashita, Kazuya; Yoshida, Tsutomu | COPYRIGHT 2001 College of American Pathologists. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan.  All inquiries regarding rights should be directed to the Gale Group.Copyright information

Background and Objective.--Abnormal protein expression and gene mutation should be examined on exactly identified lesions. To perform simultaneous analyses of oncogene or tumor suppressor gene mutations and related protein expression in single histologic sections, we have developed a novel method using an antigen-retrieval solution for a polymerase chain reaction template before immunohistochemical staining.

Methods.--Using 20 cases of sporadic colorectal carcinoma, several kinds o...

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	Novel method for simultaneous analysis of p53 and K-ras mutations and p53 protein expresssion in single histologic sections Archives of Pathology & Laboratory Medicine ; * Background and Objective.-Abnormal protein expression and gene mutation should be examined on exactly identified lesions. To perform simultaneous analyses of oncogene or tumor suppressor gene mutations and related protein expression in single histologic sections, we have developed a novel method
	Novel Method for Simultaneous Analysis of p53 and K-ras Mutations and p53 Protein Expression in Single Histologic Sections. Archives of Pathology & Laboratory Medicine ; Immunohistochemical analysis with formalin-fixed and paraffin-embedded histologic sections is frequently used in routine histopathologic diagnosis. Protein expression can thus be assessed, with better results being observed in many cases when sections are heated in an antigen-retrieval solution.
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