M30 Expression Demonstrates Apoptotic Cells, Correlates With in Situ End-Labeling, and Is Associated With Ki-67 Expression in Large Intestinal Neoplasms.

From: Archives of Pathology & Laboratory Medicine | Date: December 1, 2000| Author: Carr, Norman John | Copyright information

* Context.--The monoclonal antibody M30 recognizes a neoepitope of cytokeratin 18 produced during apoptosis. It is reactive in formalin-fixed, paraffin-embedded tissue and has great potential in the study of apoptosis in clinical and experimental material.

Objectives.--To compare the results of M30 immunoexpression with a more established technique of demonstrating apoptosis in tissue sections, in situ end-labeling. A secondary objective was to compare the results with immunoexpres...

Read all of this article with a FREE trial to HighBeam Research

Related newspaper, magazine, and journal articles from HighBeam Research

M30 expression demonstrates apoptotic cells, correlates with in situ end-labeling, and is associated with Ki-67 expression in large intestinal neoplasms
Archives of Pathology & Laboratory Medicine ; * Context-The monoclonal antibody M30 recognizes a neoepitope of cytokeratin 18 produced during apoptosis. It is reactive in formalin-fixed, paraffin-embedded tissue and has great potential in the study of apoptosis in clinical and experimental material. Objectives.-To compare the results of M30
ILISA Apoptosis Detection Kit. (Apoptosis Reagents).
Bioscience Technology ; ApoStrand[TM] ELISA Apoplosis Detection Kit is based on one-slop detection of ssDNA in apoptotic cells. It is highly specific for apoptotic cells, as ssDNA antibody will not react with necrotic cells or with cells with DNA breaks in the absence of apoptosis. Detection is bawd on the staining of
Effects of prefixation and fixation times on apoptosis detection by in situ end-labeling of fragmented DNA
Archives of Pathology & Laboratory Medicine ; * Objective.-Apoptosis is considered to play an important role in the pathogenesis and progression of neoplasia. An in situ 3'-end DNA labeling (TUNEL) method was recently developed and has been widely used to identify apoptotic cells in tissue sections. However, sometimes the TUNEL method labels
A cancer cause? (Letters).(Letter to the Editor)
Science News ; I was intrigued by the fact that some apoptotic cells can recover if not engulfed by another cell ( Get Rid of the Bodies , SN: 9/28/02, p. 202). DNA reassembly after the caspases tear it apart should result in many gene mutations. While most of the mutations would result in cell death, perhaps a
Apoptosis and Its Relevance to Autoimmunity.(Brief Article)(Book Review)
SciTech Book News ; ... apoptotic cells; MFG-E8-dependent clearance of apoptotic cells and autoimmunity caused by its failure; clearance of apoptotic cells is human systemic lupus erythematosus; and apoptosis and glomerulonephritis. ([c]20062005 Book News, Inc., Portland, OR)