Investigators at Case Western Reserve University zero in on HIV/AIDS.

AIDS Weekly | November 9, 2009 | Copyright

According to recent research published in the journal Biochemistry, "The biologically active form of HIV-1 reverse transcriptase (RT) is the p66/p51 heterodimer. The process of maturation of the heterodimer from precursor proteins is poorly understood."

"Previous Studies indicated that association of p66 and p51 is very slow. Three techniques, a pre-steady-state activity assay, intrinsic tryptophan fluorescence, and a FRET assay, were used to monitor the dimerization kinetics of RT. Kinetic experiments were conducted with purified p66 and p51 proteins in aqueous ...

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