Plant Methods

Genome-wide identification of microsatellites in white clover ( Trifolium repens L.) using FIASCO and phpSSRMiner.(Methodology)(Fast Isolation by AFLP of Sequences COntaining repeats)

Plant Methods | July 16, 2008 | Copyright

Authors: Yan Zhang [1]; Ji He [2]; Patrick X Zhao [2]; Joseph H Bouton [1]; Maria J Monteros (corresponding author) [1]

Background

Microsatellite or SSR markers are PCR-based markers widely used in mapping and QTL detection studies in plants [1]. A number of methods have been used to identify microsatellite markers including a Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) approach [2]. FIASCO is a fast and effective technique to develop SSR markers compared to methods previously used [3, 4]. Some of the advantages of FIASCO are smaller genomic DNA (250…

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