Using 454 technology for long-PCR based sequencing of the complete mitochondrial genome from single Haemonchus contortus (Nematoda).(Research article)(polymerase chain reaction)

BMC Genomics | January 11, 2008| | Copyright

Authors: Aaron R Jex (corresponding author) [1]; Min Hu [1]; D Timothy J Littlewood [2]; Andrea Waeschenbach [2]; Robin B Gasser (corresponding author) [1]

Background

The mitochondrion is the organelle responsible for cellular respiration and energy production in many eukaryotic organisms. In addition to its role in cellular function, the mitochondrion contains an internalised, usually circular genome (~13-20 kb in size) which is separate to, but co-operates with, the nuclear genome [1, 2, 3, 4]. Knowledge about mt genomes and their structure provides a ...

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