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Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model.

From: Emerging Infectious Diseases  |  Date: 11/1/2007  |  Author: Allison Lesley; Eklund, Marjut; Feng, Peter C.H.; Karch, Helge; Keen, James; Keys, Christine; Lacher, David W.; Monday, Steven R.; Nagano, Hideki; Siitonen, Anja; Whittam, Thomas S.

Escherichia coli O157:H7 variants were examined for trait mutations and by molecular subtyping to better define clonal complexes postulated on the O157:H7 evolution model. Strains of [beta]-glucuronidase--positive, sorbitol-negative O157:H7 isolated in United States and Japan were identical to A5 clonal strain and shared sequence type (ST)--65 by multilocus sequence typing (MLST); thus, they belong in A5. However, these strains exhibited pulsed-field gel electrophoresis (PFGE) ...

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